NUI Galway NBIPI cmi

Fluorescence Lifetime Imaging/FCS system (Biosciences Building)

Olympus Fluoview 1000 Confocal Microscope with Picoquant fluorescence lifetime imaging. Contrast in image is provided in terms of fluorescence lifetime rather than intensity. Possible to perform FLIM, FRET and FCS experiments.
Features
  • Inverted microscope (IX71) 
  • pulsed 488nm laser , picosecond lifetime discrimination
  • Avalanche Photo diode detection
  • standard fv1000 confocal setup - 473, 546, 633 excitation
Objectives:
  • 10 x air
  • 20x air
  • 40 x water
  • 60 x water
Light source:
  • Diode Laser System contains: pulsed 488 nm, 473 nm, 546 nm, 633 nm. epi fluorescence white light source
Filters:
  • DAPI, FITC and TRITC filter sets for epifluorescence
  • multiple filters  for a range of emission wavelengths
Scanning Modes:
  • 1 dimensional (spot scan), to 4 dimensional (x,y,z,t).
Software:
  • Fluoview 10 ASW
Contact

lsm kit

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Download FV1000 viewer (64MB)

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