Olympus Fluoview 1000 system (Biosciences Building)

Confocal Laser Scanning Microscopy (CLSM) is a fluorescence based imaging technique that provides greater resolution than conventional fluorescence microscopy. The optics are so arranged that information is collected from a very narrowly defined focal plane and out of focus “blurring” information is eliminated. Using this technique, it is possible to image thin x-y sections along the z-axis, which produces a stack of high resolution images (optical sections) devoid of out of focus light. This stack of images represents the thickness of the specimen. For example, imaging through one cell can routinely yield a stack of 20-30 optical sections. The stack of x-y sections can then be projected together to provide an extended range of focus image and rendered into 3D reconstructions.

 

Inverted microscope (IX71) fitted

Dual monitor system used to view PMT collected fluorescent/DIC images.

spectral detection, linear unmixing

Laser lines for all classes of fluorescent proteins

1 dimensional (spot scan), to 4 dimensional (x,y,z,t)

sim scanner capability

Olympus software manual