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Light microscopes use visible light sources including white light and lasers to image live and fixed samples. There are many different microscopy techniques including brightfield, phase contrast, differential interference contrast (DIC), fluorescence and confocal. The centre has a number of instruments covering these modes. Common techniques performed at the centre are listed below.

 

Confocal / Multiphoton Microscopy

Andor Revolution Spinning Disk Confocal Olympus fluoview 300
Zeiss LSM 510 Confocal with tunable Ti-Sapphire femtosecond Laser Witec Alpha 500 Raman Confocal Microscope
 
Optigrid structured illumination microscope   Olympus Fluoview 1000 BRB   Inverted confocal time resolved system    
Structured Illumination Microscope   fluoview 1000   flim fcs system    

Widefield Microscopy


Leica Teaching Microscopes
Nikon Eclipse 400   Metripol polarised light microscope
anatomy teaching microscopes Nikon Eclipse 400 upright microscope   metripol system

Live Cell Imaging

Imaging cells in real time can provide critical insights into the nature of biological cell and tissue function. At the centre we have three microscopes fitted with environmental chambers. These chambers can tightly control the temperature and humidity and CO2 levels so that living cells can be maintained in a healthy state during long term imaging experiments. The chambers are fixed and cover the whole microscope station. The Andor system is also fitted with a zero drift compensation (zdc) system to prevent focal drift during long term imaging.

Volocity Visualisation and Quantitation