Studies on the
>Photomerge of light microscopy images (taken at 20x) of human myometrium. Paraffin embedded tissue was cut and stained with Massons Trichrome Stain, this allowed us to distinguish muscle (pink-red) from collagen (green-blue). Red blood cells in vessels stain bright red. Images from these sections were used for the assessment of volume fraction of tissue composed of smooth muscle cells, nuclei and extracellular matrix.
>Scanning electron microscopy: layers of human myometrium, the smooth muscle of the uterus are pulled apart exposing collagen fibers, vessels and red blood cells.
Vessels surrounded by cross sectioned uterine smooth muscle cells.
SEM: collagen fibers surrounding smooth muscle cells of the uterus.
Photomicrograph shows a horizontal section of a Parkinsonian rat brain. Blue = DAPI stained nuclei, Green = GFAP immunostained astrocytes.
Photomicrograph shows a transverse section of a rat spinal cord with a dorsal lesion. Blue = DAPI stained nuclei, Red = NeuN immunostained neurons, Green = CD11b immunostained microglia/macrophages. Transmitted light shows outline of spinal cord section
Photomicrograph shows a transverse section of an injured rat spinal cord. Red = GFAP immunostained astrocytes, Green = vimentin immunostained reactive astrocytes. All of the images in the slides were cut on a cryostat at 20 micron thickness